Super-resolution binding activated localization microscopy through reversible change of DNA conformation
نویسندگان
چکیده
منابع مشابه
Super-Resolution Localization Microscopy
Light microscopy techniques have been vital to our understanding of biological structures and systems since their invention in the late 16 Century. However, conventional light microscopy techniques are limited by the diffraction limit of light, preventing us from seeing anything smaller than approximately 200 nm in x and y and approximately 500 nm in z. Many molecules and structures of interest...
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Traditional photon localization microscopy analyses only the spatial distributions of photons emitted by individual molecules to reconstruct super-resolution optical images. Unfortunately, however, the highly valuable spectroscopic information from these photons have been overlooked. Here we report a spectroscopic photon localization microscopy that is capable of capturing the inherent spectros...
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Kristin A. Gabor,1,2,3 Mudalige S. Gunewardene,1 David Santucci,4 and Samuel T. Hess1,3,* 1 Department of Physics and Astronomy, 120 Bennett Hall, University of Maine, Orono, ME 04469 2 Department of Molecular and Biomedical Sciences, 5735 Hitchner Hall, University of Maine, Orono, ME 04469 3 Graduate School of Biomedical Sciences, 263 ESRB/Barrows Hall, University of Maine, Orono, ME 04469 4 V...
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Super-resolution microscopy techniques, which overcome the diffraction limit of optical microscopy, are revolutionizing biological research by providing the ability to localize proteins within cells at nanometer resolution. Super-resolution localization microscopy (SRLM) is one of such techniques. It achieves nanometer resolution by randomly activating separate fluorophores and computationally ...
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Single Molecule Localization Microscopy (SMLM) is a recently emerged optical imaging method that was shown to achieve a resolution in the order of tens of nanometers in intact cells. Novel high resolution imaging methods might be crucial for understanding of how the chromatin, a complex of DNA and proteins, is arranged in the eukaryotic cell nucleus. Such an approach utilizing switching of a fl...
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ژورنال
عنوان ژورنال: Nucleus
سال: 2018
ISSN: 1949-1034,1949-1042
DOI: 10.1080/19491034.2017.1419846